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dna cdna template  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dna cdna template
    Dna Cdna Template, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+cdna+templates/DNA/pmc12681265-241-1-29
    Average 99 stars, based on 1 article reviews
    dna cdna template - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Identification of urothelial cancer-associated 1 (UCA1) as a diagnostic biomarker of pre-eclampsia via regulating microRNA-16 and its downstream signaling pathway
    Article Snippet: A miRNeasy Mini Kit (Qiagen, Hilden, Germany) was used to extract the total RNA from tissue and cell samples. .. Isolated RNA was reversely transcribed into complementary DNA (cDNA) templates using a reverse transcription kit (Thermo Fisher Scientific, Waltham, MA). .. The expression of UCA1, miR-16, and PAPP-A mRNA was measured using quantitative real-time PCR and a SYBR Premix Ex Taq II reagent kit (Takara, Tokyo, Japan) following the instructions of the kit.

    Reverse Transcription:

    Article Title: Identification of urothelial cancer-associated 1 (UCA1) as a diagnostic biomarker of pre-eclampsia via regulating microRNA-16 and its downstream signaling pathway
    Article Snippet: A miRNeasy Mini Kit (Qiagen, Hilden, Germany) was used to extract the total RNA from tissue and cell samples. .. Isolated RNA was reversely transcribed into complementary DNA (cDNA) templates using a reverse transcription kit (Thermo Fisher Scientific, Waltham, MA). .. The expression of UCA1, miR-16, and PAPP-A mRNA was measured using quantitative real-time PCR and a SYBR Premix Ex Taq II reagent kit (Takara, Tokyo, Japan) following the instructions of the kit.

    Article Title: ITGAM sustains MAPK signaling and serves as an adverse prognostic factor and therapeutic target in acute myeloid leukemia.
    Article Snippet: The sh-RNA was synthesized by Genechem (Shanghai, China). .. The transfection efficiency was verified by quantitative polymerase chain reaction (qPCR) and western blot. qPCR The cell samples were collected and total RNA was extracted with TRIzol reagent (Invitrogen, Carlsbad, CA, USA), and the complementary DNA (cDNA) templates were then synthesized using a cDNA reverse transcription kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The quantitative real-time polymerase chain reaction (qRTPCR) was performed by the ABI Prism 7500 system (Applied Biosystems, Foster City, CA, USA) using SYBR Green Supermix (Invitrogen, Carlsbad, CA, USA) kit.

    Article Title: RapaNet: A Web Tool for the Co-Expression Analysis of Brassica rapa Genes
    Article Snippet: The total RNA from representative organs, such as the leaf, root, and flower, was extracted using the TRI Reagent (Molecular Research Center, www.mrcgene.com ) and cleaned with the Qiagen RNeasy mini kit (Qiagen, www.qiagen.com ). .. The complementary DNA (cDNA) templates were synthesized using the RevertAid H Minus M-MulV reverse transcriptase (Fermentas, www.fermentas.com ). ..

    Article Title: Early single session of hyperbaric oxygen therapy mitigates renal apoptosis in lipopolysaccharides-induced endotoxemia in rats
    Article Snippet: RNA concentrations were ascertained by measuring absorbance at 260 nm (Ultraspec 2000; Pharmacia Biotech). .. The messenger RNA (mRNA) expressions of genes associated with inflammation and fibrosis, including interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), monocyte chemoattractant protein 1 (MCP1), and alpha smooth muscle actin (α-SMA), were assessed through real-time PCR utilizing complementary DNA (cDNA) templates. cDNA was synthesized by reverse transcribing 5 μg of total RNA using oligo (dT) priming and SuperScript II Reverse Transcriptase (Invitrogen). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Upregulated miR-193a-3p as an oncogene in esophageal squamous cell carcinoma regulating cellular proliferation, migration and apoptosis
    Article Snippet: RNA isolation and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) According to the manufacturer's protocol, total RNA was extracted with TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and purified with RNeasy Maxi kit (Qiagen GmbH). .. Total RNA (1 μg of each sample) was used for RT using miScript RT kit (Qiagen GmbH) to obtain the complementary DNA (cDNA) templates. qPCR reaction of miR-193a-3p was performed in an ABI PRISM ® 7000 Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific, Inc.) using miScript SYBR Green PCR kit (Qiagen GmbH). ..

    Article Title: ITGAM sustains MAPK signaling and serves as an adverse prognostic factor and therapeutic target in acute myeloid leukemia.
    Article Snippet: The sh-RNA was synthesized by Genechem (Shanghai, China). .. The transfection efficiency was verified by quantitative polymerase chain reaction (qPCR) and western blot. qPCR The cell samples were collected and total RNA was extracted with TRIzol reagent (Invitrogen, Carlsbad, CA, USA), and the complementary DNA (cDNA) templates were then synthesized using a cDNA reverse transcription kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The quantitative real-time polymerase chain reaction (qRTPCR) was performed by the ABI Prism 7500 system (Applied Biosystems, Foster City, CA, USA) using SYBR Green Supermix (Invitrogen, Carlsbad, CA, USA) kit.

    Article Title: Early single session of hyperbaric oxygen therapy mitigates renal apoptosis in lipopolysaccharides-induced endotoxemia in rats
    Article Snippet: RNA concentrations were ascertained by measuring absorbance at 260 nm (Ultraspec 2000; Pharmacia Biotech). .. The messenger RNA (mRNA) expressions of genes associated with inflammation and fibrosis, including interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), monocyte chemoattractant protein 1 (MCP1), and alpha smooth muscle actin (α-SMA), were assessed through real-time PCR utilizing complementary DNA (cDNA) templates. cDNA was synthesized by reverse transcribing 5 μg of total RNA using oligo (dT) priming and SuperScript II Reverse Transcriptase (Invitrogen). ..

    SYBR Green Assay:

    Article Title: Upregulated miR-193a-3p as an oncogene in esophageal squamous cell carcinoma regulating cellular proliferation, migration and apoptosis
    Article Snippet: RNA isolation and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) According to the manufacturer's protocol, total RNA was extracted with TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and purified with RNeasy Maxi kit (Qiagen GmbH). .. Total RNA (1 μg of each sample) was used for RT using miScript RT kit (Qiagen GmbH) to obtain the complementary DNA (cDNA) templates. qPCR reaction of miR-193a-3p was performed in an ABI PRISM ® 7000 Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific, Inc.) using miScript SYBR Green PCR kit (Qiagen GmbH). ..

    Polymerase Chain Reaction:

    Article Title: Upregulated miR-193a-3p as an oncogene in esophageal squamous cell carcinoma regulating cellular proliferation, migration and apoptosis
    Article Snippet: RNA isolation and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) According to the manufacturer's protocol, total RNA was extracted with TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and purified with RNeasy Maxi kit (Qiagen GmbH). .. Total RNA (1 μg of each sample) was used for RT using miScript RT kit (Qiagen GmbH) to obtain the complementary DNA (cDNA) templates. qPCR reaction of miR-193a-3p was performed in an ABI PRISM ® 7000 Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific, Inc.) using miScript SYBR Green PCR kit (Qiagen GmbH). ..

    Article Title: Endometrial Injury and Its Rescue by Mesenchymal Stem Cells Is Dependent on Estrous Cycle Phase
    Article Snippet: Total RNA was extracted from endometrial tissues with Trizol reagent (Ambion, Quantity, Grand Island, New York) according to the manufacturer's instructions. .. The complementary DNA (cDNA) templates for PCR analysis were synthesised from the total RNA following the instructions of the first‐strand cDNA synthesis kit (Fermentas, Toronto, Canada). .. Real‐time PCR was performed using SYBR Green PCR Master Mix Reagent (SYBR Premix Ex Taq kit, Cat. DRR041A; TaKaRa Clontech, Mountain View, California) and the LightCycler 480 SYBR Green I Master (Roche Diagnostics International Ltd).

    Transfection:

    Article Title: ITGAM sustains MAPK signaling and serves as an adverse prognostic factor and therapeutic target in acute myeloid leukemia.
    Article Snippet: The sh-RNA was synthesized by Genechem (Shanghai, China). .. The transfection efficiency was verified by quantitative polymerase chain reaction (qPCR) and western blot. qPCR The cell samples were collected and total RNA was extracted with TRIzol reagent (Invitrogen, Carlsbad, CA, USA), and the complementary DNA (cDNA) templates were then synthesized using a cDNA reverse transcription kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The quantitative real-time polymerase chain reaction (qRTPCR) was performed by the ABI Prism 7500 system (Applied Biosystems, Foster City, CA, USA) using SYBR Green Supermix (Invitrogen, Carlsbad, CA, USA) kit.

    Western Blot:

    Article Title: ITGAM sustains MAPK signaling and serves as an adverse prognostic factor and therapeutic target in acute myeloid leukemia.
    Article Snippet: The sh-RNA was synthesized by Genechem (Shanghai, China). .. The transfection efficiency was verified by quantitative polymerase chain reaction (qPCR) and western blot. qPCR The cell samples were collected and total RNA was extracted with TRIzol reagent (Invitrogen, Carlsbad, CA, USA), and the complementary DNA (cDNA) templates were then synthesized using a cDNA reverse transcription kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The quantitative real-time polymerase chain reaction (qRTPCR) was performed by the ABI Prism 7500 system (Applied Biosystems, Foster City, CA, USA) using SYBR Green Supermix (Invitrogen, Carlsbad, CA, USA) kit.

    Synthesized:

    Article Title: ITGAM sustains MAPK signaling and serves as an adverse prognostic factor and therapeutic target in acute myeloid leukemia.
    Article Snippet: The sh-RNA was synthesized by Genechem (Shanghai, China). .. The transfection efficiency was verified by quantitative polymerase chain reaction (qPCR) and western blot. qPCR The cell samples were collected and total RNA was extracted with TRIzol reagent (Invitrogen, Carlsbad, CA, USA), and the complementary DNA (cDNA) templates were then synthesized using a cDNA reverse transcription kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The quantitative real-time polymerase chain reaction (qRTPCR) was performed by the ABI Prism 7500 system (Applied Biosystems, Foster City, CA, USA) using SYBR Green Supermix (Invitrogen, Carlsbad, CA, USA) kit.

    Article Title: RapaNet: A Web Tool for the Co-Expression Analysis of Brassica rapa Genes
    Article Snippet: The total RNA from representative organs, such as the leaf, root, and flower, was extracted using the TRI Reagent (Molecular Research Center, www.mrcgene.com ) and cleaned with the Qiagen RNeasy mini kit (Qiagen, www.qiagen.com ). .. The complementary DNA (cDNA) templates were synthesized using the RevertAid H Minus M-MulV reverse transcriptase (Fermentas, www.fermentas.com ). ..

    Article Title: Early single session of hyperbaric oxygen therapy mitigates renal apoptosis in lipopolysaccharides-induced endotoxemia in rats
    Article Snippet: RNA concentrations were ascertained by measuring absorbance at 260 nm (Ultraspec 2000; Pharmacia Biotech). .. The messenger RNA (mRNA) expressions of genes associated with inflammation and fibrosis, including interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), monocyte chemoattractant protein 1 (MCP1), and alpha smooth muscle actin (α-SMA), were assessed through real-time PCR utilizing complementary DNA (cDNA) templates. cDNA was synthesized by reverse transcribing 5 μg of total RNA using oligo (dT) priming and SuperScript II Reverse Transcriptase (Invitrogen). ..

    cDNA Synthesis:

    Article Title: Endometrial Injury and Its Rescue by Mesenchymal Stem Cells Is Dependent on Estrous Cycle Phase
    Article Snippet: Total RNA was extracted from endometrial tissues with Trizol reagent (Ambion, Quantity, Grand Island, New York) according to the manufacturer's instructions. .. The complementary DNA (cDNA) templates for PCR analysis were synthesised from the total RNA following the instructions of the first‐strand cDNA synthesis kit (Fermentas, Toronto, Canada). .. Real‐time PCR was performed using SYBR Green PCR Master Mix Reagent (SYBR Premix Ex Taq kit, Cat. DRR041A; TaKaRa Clontech, Mountain View, California) and the LightCycler 480 SYBR Green I Master (Roche Diagnostics International Ltd).



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